Seminar On Fluorescence Spectroscopy

Fluorescence Spectroscopy Seminar Report and PPT

Fluorescence is a spectrochemical method of analysis where the molecules of the analyte are excited by irradiation at a certain wavelength and emit radiation of a different wavelength. The emission spectrum provides information for both qualitative and quantitative analysis.When light of an appropriate wavelength is absorbed by a molecule (i.e., excitation), the electronic state of the molecule changes from the ground state to one of many vibrational levels in one of the excited electronic states. The excited electronic state is usually the first excited singlet state.Once the molecule is in this excited state, relaxation can occur via several processes. Fluorescence is one of these processes and results in the emission of light.

1. Excitation and Emission Spectra of Fluorescein
2. Cell Handling
3. Emission Spectrum
4. Excitation Spectrum
5. Inner Filter Effect
6. Bandwidth Effect on the Quality of the Spectrum
7. Analysis of Quinine in Tonic Water- Calibration Curve Method

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